Células-tronco pluripotentes humanamente induzidas livres de vetor e sequências transgênicas

Science 8 May 2009:
Vol. 324. no. 5928, pp. 797 - 801
DOI: 10.1126/science.1172482

Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences

Junying Yu,1,2,3,* Kejin Hu,3 Kim Smuga-Otto,1,2,3 Shulan Tian,1,2 Ron Stewart,1,2 Igor I. Slukvin,3,4 James A. Thomson1,2,3,5,*

Reprogramming differentiated human cells to induced pluripotent stem (iPS) cells has applications in basic biology, drug development, and transplantation. Human iPS cell derivation previously required vectors that integrate into the genome, which can create mutations and limit the utility of the cells in both research and clinical applications. We describe the derivation of human iPS cells with the use of nonintegrating episomal vectors. After removal of the episome, iPS cells completely free of vector and transgene sequences are derived that are similar to human embryonic stem (ES) cells in proliferative and developmental potential. These results demonstrate that reprogramming human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors and removes one obstacle to the clinical application of human iPS cells.

1 Morgridge Institute for Research, Madison, WI 53707–7365, USA.

2 Genome Center of Wisconsin, Madison, WI 53706–1580, USA.

3 Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI 53715–1299, USA.

4 Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA.

5 Department of Anatomy, University of Wisconsin-Madison, Madison, WI 53706–1509, USA.

* To whom correspondence should be addressed. E-mail: jyyu2008@gmail.com (J.Y.); thomson@primate.wisc.edu (J.A.T.)

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A cada dia que passa, mais forte fica a favor o uso das células-tronco adultas do que a nota promissória das células-tronco embrionárias e toda sua complicação ética e de tratamento nada democrático (que a turma do andar de cima e a Grande Mídia tupiniquim nunca colocaram na mesa para debate). Não fica por menos de US$ 1.000.000,00 (Um milhão de dólares) clonar para fins terapêuticos. Fora as complicações colaterais para as mulheres que tiverem seus óvulos retirados cirurgicamente. Por que eles não falam sobre isso?