Genoma de Naegleri gruberi lança luz sobre antiga versatilidade eucariótica

Cell
Volume 140, Issue 5, 5 March 2010, Pages 631-642

The Genome of Naegleria gruberi Illuminates Early Eukaryotic Versatility

Lillian K. Fritz-Laylin1, 10, Simon E. Prochnik3, 10, Michael L. Ginger4, Joel B. Dacks5, 6, Meredith L. Carpenter1, Mark C. Field6, Alan Kuo3, Alex Paredez1, Jarrod Chapman3, Jonathan Pham7, Shengqiang Shu3, Rochak Neupane2, Michael Cipriano7, Joel Mancuso8, Hank Tu3, 11, Asaf Salamov3, Erika Lindquist3, Harris Shapiro3, Susan Lucas3, Igor V. Grigoriev3, W. Zacheus Cande1, Chandler Fulton9, Daniel S. Rokhsar1, 3,, and Scott C. Dawson7, ,

1 Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA

2 Center for Integrative Genomics, 545 Life Sciences Addition, University of California, Berkeley, Berkeley, CA 94720, USA

3 U.S. Department of Energy, Joint Genome Institute, Walnut Creek, CA 94598, USA

4 School of Health and Medicine, Division of Biomedical and Life Sciences, Lancaster University, Lancaster LA1 4YQ, UK

5 Department of Cell Biology, University of Alberta Edmonton, Alberta, Canada

6 The Molteno Building, Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK

7 Department of Microbiology, University of California, Davis, CA 95616, USA

8 Gatan Inc., 5794 W. Las Positas Boulevard, Pleasanton, CA 94588, USA

9 Department of Biology, Brandeis University, Waltham, MA 02454-9110, USA

Received 28 August 2009;
revised 17 November 2009;
accepted 15 January 2010.
Published: March 4, 2010.
Available online 4 March 2010.

Referred to by: The Incredible Expanding Ancestor of Eukaryotes
Cell, Volume 140, Issue 5, 5 March 2010, Pages 606-608,
Eugene V. Koonin
 PDF (496 K)

Summary

Genome sequences of diverse free-living protists are essential for understanding eukaryotic evolution and molecular and cell biology. The free-living amoeboflagellate Naegleria gruberi belongs to a varied and ubiquitous protist clade (Heterolobosea) that diverged from other eukaryotic lineages over a billion years ago. Analysis of the 15,727 protein-coding genes encoded by Naegleria's 41 Mb nuclear genome indicates a capacity for both aerobic respiration and anaerobic metabolism with concomitant hydrogen production, with fundamental implications for the evolution of organelle metabolism. The Naegleria genome facilitates substantially broader phylogenomic comparisons of free-living eukaryotes than previously possible, allowing us to identify thousands of genes likely present in the pan-eukaryotic ancestor, with 40% likely eukaryotic inventions. Moreover, we construct a comprehensive catalog of amoeboid-motility genes. The Naegleria genome, analyzed in the context of other protists, reveals a remarkably complex ancestral eukaryote with a rich repertoire of cytoskeletal, sexual, signaling, and metabolic modules.

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