Um código de barras de DNA para plantas terrestrs

A DNA barcode for land plants

CBOL Plant Working Group1

+Author Affiliations

Communicated by Daniel H. Janzen, University of Pennsylvania, Philadelphia, PA, May 27, 2009 (received for review March 18, 2009)

Peter M. Hollingswortha,2, Laura L. Forresta, John L. Spougeb, Mehrdad Hajibabaeic, Sujeevan Ratnasinghamc, Michelle van der Bankd, Mark W. Chasee, Robyn S. Cowane, David L. Ericksonf, Aron J. Fazekasg, Sean W. Grahamh, Karen E. Jamesi, Ki-Joong Kimj, W. John Kressf, Harald Schneideri, Jonathan van AlphenStahle, Spencer C.H. Barrettk, Cassio van den Bergl, Diego Bogarinm, Kevin S. Burgessk,n, Kenneth M. Camerono, Mark Carinei, Juliana Chacónp, Alexandra Clarka, James J. Clarksone, Ferozah Conradq, Dion S. Deveye, Caroline S. Fordr, Terry A.J. Heddersons, Michelle L. Hollingswortha, Brian C. Husbandg, Laura J. Kellya,e, Prasad R. Kesanakurtig, Jung Sung Kimj, Young-Dong Kimt, Renaud Lahayed, Hae-Lim Leej, David G. Longa, Santiago Madriñánp, Olivier Maurind, Isabelle Meusnierc, Steven G. Newmasterg, Chong-Wook Parku, Diana M. Percyh, Gitte Petersenv, James E. Richardsona, Gerardo A. Salazarw, Vincent Savolainene,x, Ole Sebergv, Michael J. Wilkinsonr, Dong-Keun Yij and Damon P. Littley

+Author Affiliations

aRoyal Botanic Garden Edinburgh, Edinburgh EH3 5LR, United Kingdom;

bNational Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Computational Biology Branch, Bethesda, MD 20894;

cBiodiversity Institute of Ontario, Department of Integrative Biology, University of Guelph, Guelph, ON, Canada N1G 2W1;

dDepartment of Botany and Plant Biotechnology, University of Johannesburg, P.O. Box 524, Auckland Park, Johannesburg 2006, South Africa;

eRoyal Botanic Gardens, Kew, Richmond TW9 3DS, United Kingdom;

fDepartment of Botany, Smithsonian Institution, Washington DC, 20013-7012;

gDepartment of Integrative Biology, University of Guelph, Guelph, ON, Canada N1G 2W1;

hUBC Botanical Garden and Centre for Plant Research, Faculty of Land and Food Systems, and Department of Botany, University of British Columbia, Vancouver, BC, Canada V6T 1Z4;

iBotany Department, Natural History Museum, London SW7 5BD, United Kingdom;

jSchool of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea;

kDepartment of Ecology and Evolutionary Biology, University of Toronto, Toronto, ON, Canada M5S 3B2;

lLaboratório de Sistemática Molecular de Plantas, Universidade Estadual de Feira de Santana, Departamento de Ciências Biológicas, 44031-460, Feira de Santana, Bahia, Brazil;

mJardín Botánico Lankester, Universidad de Costa Rica, Cartago, Costa Rica;

nDepartment of Biology, Columbus State University, Columbus, GA 31907-5645;

oDepartment of Botany, University of Wisconsin, Madison, WI 53508;

pUniversidad de los Andes, Apartado Aéreo 4976, Bogotá, D.C., Colombia;

qLeslie Hill Molecular Systematics Laboratory, SANBI, Kirstenbosch Research Centre, Claremont 7735, Cape Town, South Africa;

rInstitute of Biological, Environmental and Rural Sciences, Aberystwyth University, Ceredigion SY23 3DA, United Kingdom;

sDepartment of Botany, University of Cape Town, Rondebosch 7700, South Africa;

tDepartment of Life Sciences, Hallym University, Chuncheon 200-702, Korea;

uSchool of Biological Sciences, Seoul National University, Seoul 151-742, Korea;

vNatural History Museum of Denmark, University of Copenhagen, 1307 Copenhagen K, Denmark;

wInstituto de Biología, Universidad Nacional Autónoma de México, 04510 México, D.F., Mexico;

xImperial College London, Silwood Park Campus, Ascot SL5 7PY, United Kingdom; and

yCullman Program for Molecular Systematics, New York Botanical Garden, Bronx, NY, 10458-5126

Communicated by Daniel H. Janzen, University of Pennsylvania, Philadelphia, PA, May 27, 2009 (received for review March 18, 2009)

Abstract

DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7 leading candidate plastid DNA regions (atpF–atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 gene, psbK–psbI spacer, and trnH–psbA spacer). Based on assessments of recoverability, sequence quality, and levels of species discrimination, we recommend the 2-locus combination of rbcL+matK as the plant barcode. This core 2-locus barcode will provide a universal framework for the routine use of DNA sequence data to identify specimens and contribute toward the discovery of overlooked species of land plants.

matK rbcL species identification

Footnotes

2To whom correspondence should be addressed. E-mail: P.Hollingsworth@rbge.org.uk
Author contributions: P.M.H., L.L.F., J.L.S., M.H., S.R., M.v.d.B., M.W.C., R.S.C., D.L.E., A.J.F., S.W.G., K.E.J., K.-J.K., W.J.K., H.S., S.C.H.B., C.v.d.B., M.C., T.A.J.H., B.C.H., G.P., J.E.R., G.A.S., V.S., O.S., M.J.W., and D.P.L. designed research; D.L.E., A.J.F., K.E.J., J.v.A.S., D.B., K.S.B., K.M.C., J.C., A.C., J.J.C., F.C., D.S.D., C.S.F., M.L.H., L.J.K., P.R.K., J.S.K., Y.D.K., R.L., H.-L.L., D.G.L., S.M., O.M., I.M., S.G.N., C.-W.P., D.M.P., and D.-K.Y. performed research; L.L.F., J.L.S., M.H., S.R., and D.P.L. analyzed data; and P.M.H., S.W.G., S.C.H.B., and D.P.L. wrote the paper.

Conflict of interest statement: Following the publication of Lahaye et al. (PNAS 105:2923, 2008), the process of filing a patent on DNA barcoding of land plants using matK was initiated by V.S., M.v.d.B., R.L., and D.B., but because of the lack of commercial interest the patent application was subsequently dropped.

Freely available online through the PNAS open access option.

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