A CRISPR view of development
Melissa M. Harrison 1, Brian V. Jenkins 2, Kate M. O’Connor-Giles 3,4 and Jill Wildonger 2
- Author Affiliations
1 Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53706, USA;
2 Biochemistry Department, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA;
3 Laboratory of Genetics,
4 Laboratory of Cell and Molecular Biology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA
Corresponding author: wildonger@wisc.edu
Abstract
The CRISPR (clustered regularly interspaced short palindromic repeat)–Cas9 (CRISPR-associated nuclease 9) system is poised to transform developmental biology by providing a simple, efficient method to precisely manipulate the genome of virtually any developing organism. This RNA-guided nuclease (RGN)-based approach already has been effectively used to induce targeted mutations in multiple genes simultaneously, create conditional alleles, and generate endogenously tagged proteins. Illustrating the adaptability of RGNs, the genomes of >20 different plant and animal species as well as multiple cell lines and primary cells have been successfully modified. Here we review the current and potential uses of RGNs to investigate genome function during development.
Keywords CRISPR Cas9 development genome editing genome engineering RNA-guided nuclease
Footnotes
Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.248252.114.
Freely available online through the Genes & Development Open Access option.
© 2014 Harrison et al.; Published by Cold Spring Harbor Laboratory Press
This article, published in Genes & Development, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.
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