Studies of G-quadruplexes formed within self-assembled DNA mini-circles
Ramon Vilar, Beata Klejevskaja, Alice Pyne, Matthew T Reynolds, Arun Shivalingam, Richard Thorogate, Bart Hogenboom and Liming Ying
Chem. Commun., 2016, Accepted Manuscript
DOI: 10.1039/C6CC07110D
Received 30 Aug 2016, Accepted 22 Sep 2016
First published online 23 Sep 2016 This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.
Abstract
We have developed self-assembled DNA mini-circles that contain a G-quadruplex-forming sequence from the c-Myc oncogene promoter and demonstrate by FRET that the G-quadruplex unfolding kinetics are 10-fold slower than for the simpler 24-mer G-quadruplex that is commonly used for FRET experiments.
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