Variation in the molecular clock of primates
Priya Moorjani a,b,1,2, Carlos Eduardo G. Amorim a,1, Peter F. Arndt c, and Molly Przeworski a,d,2
Author Affiliations
aDepartment of Biological Sciences, Columbia University, New York, NY 10027;
bProgram in Medical and Population Genetics, Broad Institute of MIT and Harvard, Cambridge, MA 02142;
cDepartment of Computational Molecular Biology, Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany;
dDepartment of Systems Biology, Columbia University, New York, NY 10027
Edited by David C. Page, Whitehead Institute, Cambridge, MA, and approved July 19, 2016 (received for review January 8, 2016)
Fig. 1. Phylogenetic tree for 10 primates. Autosomal neutral substitution rates for 10 primates and an outgroup (mouse, shown in gray) from the Multiz dataset were estimated using Phylofit (see SI Appendix, Note S1 for details of dataset and filtering). Branch lengths reflect the expected number of neutral substitutions per site along each lineage. R code to replicate this figure is available at: https://github.com/priyamoorjani/Molecular-clock_figures-and-data/blob/master/Figure1.R.
Significance
Much of our understanding of the chronology of human evolution relies on a fixed “molecular clock”; that is, a constant rate of substitutions per unit time. To evaluate the validity of this assumption, we analyze whole-genome sequences from 10 primate species. We find that there is substantial variation in the molecular clock between apes and monkeys and that rates even differ within hominines. Importantly, not all mutation types behave similarly; notably, transitions at CpG sites exhibit a more clocklike behavior than other substitutions, presumably because of their nonreplicative origin. Thus, the mutation spectra, and not just the overall substitution rates, are changing across primates. This finding suggests that events in primate evolution are most reliably dated using CpG transitions.
Abstract
Events in primate evolution are often dated by assuming a constant rate of substitution per unit time, but the validity of this assumption remains unclear. Among mammals, it is well known that there exists substantial variation in yearly substitution rates. Such variation is to be expected from differences in life history traits, suggesting it should also be found among primates. Motivated by these considerations, we analyze whole genomes from 10 primate species, including Old World Monkeys (OWMs), New World Monkeys (NWMs), and apes, focusing on putatively neutral autosomal sites and controlling for possible effects of biased gene conversion and methylation at CpG sites. We find that substitution rates are up to 64% higher in lineages leading from the hominoid–NWM ancestor to NWMs than to apes. Within apes, rates are ∼2% higher in chimpanzees and ∼7% higher in the gorilla than in humans. Substitution types subject to biased gene conversion show no more variation among species than those not subject to it. Not all mutation types behave similarly, however; in particular, transitions at CpG sites exhibit a more clocklike behavior than do other types, presumably because of their nonreplicative origin. Thus, not only the total rate, but also the mutational spectrum, varies among primates. This finding suggests that events in primate evolution are most reliably dated using CpG transitions. Taking this approach, we estimate the human and chimpanzee divergence time is 12.1 million years, and the human and gorilla divergence time is 15.1 million years.
molecular clock mutation rate primate evolution CpG transition rate human–ape divergence time
Footnotes
1P.M. and C.E.G.A. contributed equally to this work.
2To whom correspondence may be addressed. Email: pm2730@columbia.edu or mp3284@columbia.edu.
Author contributions: P.M., C.E.G.A., and M.P. designed research; P.M., C.E.G.A., and M.P. performed research; P.M. and P.F.A. contributed new reagents/analytic tools; P.M., C.E.G.A., and M.P. analyzed data; and P.M., C.E.G.A., and M.P. wrote the paper.
The authors declare no conflict of interest.
This article is a PNAS Direct Submission.
This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1600374113/-/DCSupplemental.
Freely available online through the PNAS open access option.
FREE PDF GRATIS: PNAS
