Microscopia inovadora desbloqueia segredos da montagem de vírus de plantas

sábado, dezembro 12, 2015


Mechanisms of assembly and genome packaging in an RNA virus revealed by high-resolution cryo-EM

Emma L. Hesketh, Yulia Meshcheriakova, Kyle C. Dent, Pooja Saxena, Rebecca F. Thompson, Joseph J. Cockburn, George P. Lomonossoff & Neil A. Ranson

Affiliations Contributions Corresponding author

Nature Communications 6, Article number: 10113 doi:10.1038/ncomms10113

Received 06 August 2015 Accepted 03 November 2015 Published 10 December 2015


Cowpea mosaic virus is a plant-infecting member of the Picornavirales and is of major interest in the development of biotechnology applications. Despite the availability of >100 crystal structures of Picornavirales capsids, relatively little is known about the mechanisms of capsid assembly and genome encapsidation. Here we have determined cryo-electron microscopy reconstructions for the wild-type virus and an empty virus-like particle, to 3.4 Å and 3.0 Å resolution, respectively, and built de novo atomic models of their capsids. These new structures reveal the C-terminal region of the small coat protein subunit, which is essential for virus assembly and which was missing from previously determined crystal structures, as well as residues that bind to the viral genome. These observations allow us to develop a new model for genome encapsidation and capsid assembly.

Subject terms: Biological sciences Virology Biotechnology

FREE PDF GRATIS: Nature Communications

                            Sup. Info.