A transcrição embriônica é controlada pelo estado da cromatina definido maternalmente

domingo, dezembro 20, 2015

Embryonic transcription is controlled by maternally defined chromatin state

Saartje Hontelez, Ila van Kruijsbergen, Georgios Georgiou, Simon J. van Heeringen, Ozren Bogdanovic, Ryan Lister & Gert Jan C. Veenstra

Affiliations Contributions Corresponding author

Nature Communications 6, Article number: 10148 doi:10.1038/ncomms10148

Received 12 May 2015 Accepted 10 November 2015 Published 18 December 2015

Article tools


Histone-modifying enzymes are required for cell identity and lineage commitment, however little is known about the regulatory origins of the epigenome during embryonic development. Here we generate a comprehensive set of epigenome reference maps, which we use to determine the extent to which maternal factors shape chromatin state in Xenopus embryos. Using α-amanitin to inhibit zygotic transcription, we find that the majority of H3K4me3- and H3K27me3-enriched regions form a maternally defined epigenetic regulatory space with an underlying logic of hypomethylated islands. This maternal regulatory space extends to a substantial proportion of neurula stage-activated promoters. In contrast, p300 recruitment to distal regulatory regions requires embryonic transcription at most loci. The results show that H3K4me3 and H3K27me3 are part of a regulatory space that exerts an extended maternal control well into post-gastrulation development, and highlight the combinatorial action of maternal and zygotic factors through proximal and distal regulatory sequences.

Subject terms: Biological sciences Developmental biology Molecular biology

FREE PDF GRATIS: Nature Communications