Pesquisas de G-quadruplexos formados dentro de mini círculos de DNA automontados

quinta-feira, setembro 29, 2016

Studies of G-quadruplexes formed within self-assembled DNA mini-circles

Ramon Vilar, Beata Klejevskaja, Alice Pyne, Matthew T Reynolds, Arun Shivalingam, Richard Thorogate, Bart Hogenboom and Liming Ying 

Chem. Commun., 2016, Accepted Manuscript

Received 30 Aug 2016, Accepted 22 Sep 2016

First published online 23 Sep 2016 This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.


We have developed self-assembled DNA mini-circles that contain a G-quadruplex-forming sequence from the c-Myc oncogene promoter and demonstrate by FRET that the G-quadruplex unfolding kinetics are 10-fold slower than for the simpler 24-mer G-quadruplex that is commonly used for FRET experiments.