Transcriptional accuracy modeling suggests two-step proofreading by RNA polymerase
Harriet Mellenius Måns Ehrenberg
Nucleic Acids Research, gkx849, https://doi.org/10.1093/nar/gkx849
Published: 28 September 2017
Article history
Received: 04 August 2016 Revision Received: 10 September 2017
Accepted: 22 September 2017
Source/Fonte: Nucleic Acids Research
Abstract
We suggest a novel two-step proofreading mechanism with two sequential rounds of proofreading selection in mRNA transcription. It is based on the previous experimental observations that the proofreading RNA polymerase cleaves off transcript fragments of at least 2 nt and that transcript elongation after a nucleotide misincorporation is anomalously slow. Taking these results into account, we extend the description of the accuracy of template guided nucleotide selection beyond previous models of RNA polymerase-dependent DNA transcription. The model derives the accuracy of initial and proofreading base selection from experimentally estimated nearest-neighbor parameters. It is also used to estimate the small accuracy enhancement of polymerase revisiting of previous positions following transcript cleavage.
Issue Section: COMPUTATIONAL BIOLOGY
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