Cryobiology
Volume 72, Issue 1, February 2016, Pages 78–81
Recovery and reproduction of an Antarctic tardigrade retrieved from a moss sample frozen for over 30 years
Megumu Tsujimoto a, Satoshi Imura a, b, Hiroshi Kanda a
a National Institute of Polar Research (NIPR), 10-3 Midori-cho, Tachikawa-shi, Tokyo 190-8518, Japan
b SOKENDAI (The Graduate University for Advanced Studies), Tokyo, Japan
Received 9 November 2015, Revised 22 December 2015, Accepted 22 December 2015, Available online 25 December 2015
Under a Creative Commons license
Source/Fonte: National Geographic
Abstract
Long-term survival has been one of the most studied of the extraordinary physiological characteristics of cryptobiosis in micrometazoans such as nematodes, tardigrades and rotifers. In the available studies of long-term survival of micrometazoans, instances of survival have been the primary observation, and recovery conditions of animals or subsequent reproduction are generally not reported. We therefore documented recovery conditions and reproduction immediately following revival of tardigrades retrieved from a frozen moss sample collected in Antarctica in 1983 and stored at −20 °C for 30.5 years. We recorded recovery of two individuals and development of a separate egg of the Antarctic tardigrade, Acutuncus antarcticus, providing the longest records of survival for tardigrades as animals or eggs. One of the two resuscitated individuals and the hatchling successfully reproduced repeatedly after their recovery from long-term cryptobiosis. This considerable extension of the known length of long-term survival of tardigrades recorded in our study is interpreted as being associated with the minimum oxidative damage likely to have resulted from storage under stable frozen conditions. The long recovery times of the revived tardigrades observed is suggestive of the requirement for repair of damage accrued over 30 years of cryptobiosis. Further more detailed studies will improve understanding of mechanisms and conditions underlying the long-term survival of cryptobiotic organisms.
Keywords Long-term survival; Cryptobiosis; Cryobiosis; Freezing; Acutuncus antarcticus
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