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Antimicrob Agents Chemother. 2004 March; 48(3): 1032–1033.
doi: 10.1128/AAC.48.3.1032-1033.2004.

Copyright © 2004, American Society for Microbiology

In Vitro Evolution Predicts that the IMP-1 Metallo-β-Lactamase Does Not Have the Potential To Evolve Increased Activity against Imipenem [PDF gratuito]

Barry G. Hall*

Biology Department, University of Rochester, Rochester, New York
*E-mail: drbh@mail.rochester.edu

Received July 31, 2003; Revised October 20, 2003; Accepted November 11, 2003.

ABSTRACT

In vitro evolution was used to predict whether the IMP-1 metallo-β-lactamase has the potential to evolve an increased ability to confer resistance to imipenem. Screening of eight libraries containing 9.8 × 106 ± 1.4 × 106 (mean ± standard error) variants per library, with an average of 1.2 mutations per variant, detected no increased resistance to imipenem. The results predict, with >99.9% confidence, that even under intense selection the IMP-1 β-lactamase will not evolve to confer increased resistance to imipenem.