Annita Louloupi5, Evgenia Ntini5, Thomas Conrad, Ulf Andersson Vang Ørom6
5These authors contributed equally
6Lead Contact
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DOI: https://doi.org/10.1016/j.celrep.2018.05.077 |
Article Info
Publication History
Published: June 19, 2018 Accepted: May 23, 2018
Received in revised form: April 30, 2018 Received: January 10, 2018
User License
Creative Commons Attribution – NonCommercial – NoDerivs (CC BY-NC-ND 4.0)
Source/Fonte: Ulf Andersson Vang Ørom
Highlights
•A time-resolved high-resolution picture of m6A on nascent RNA transcripts
•m6A is deposited at nascent RNA and in introns
•m6A deposition at splice-junctions increases splicing kinetics
•High m6A levels in introns is associated with slow and alternative splicing
Summary
Splicing efficiency varies among transcripts, and tight control of splicing kinetics is crucial for coordinated gene expression. N-6-methyladenosine (m6A) is the most abundant RNA modification and is involved in regulation of RNA biogenesis and function. The impact of m6A on regulation of RNA splicing kinetics is unknown. Here, we provide a time-resolved high-resolution assessment of m6A on nascent RNA transcripts and unveil its importance for the control of RNA splicing kinetics. We find that early co-transcriptional m6A deposition near splice junctions promotes fast splicing, while m6A modifications in introns are associated with long, slowly processed introns and alternative splicing events. In conclusion, we show that early m6A deposition specifies the fate of transcripts regarding splicing kinetics and alternative splicing.
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