Clonagem de macacos através de transferência nuclear de células somáticas - clonagem humana à vista?

Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer

Zhen Liu, Yijun Cai, Yan Wang, Yanhong Nie, Chenchen Zhang, Yuting Xu, Xiaotong Zhang, Yong Lu, Zhanyang Wang, Muming Poo, Qiang Sun 

Correspondence

Corresponding author2, Qiang Sun
2Lead Contact

Published: January 24, 2018
Publication stage: In Press Corrected Proof

Cloning of Macaque Monkeys by Somatic Cell Nuclear Transfer
DOI: http://dx.doi.org/10.1016/j.cell.2018.01.020

Article Info

Publication History
Published: January 24, 2018 Accepted: January 16, 2018; Received in revised form: January 11, 2018; Received: December 21, 2017

Highlights

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  Somatic cell nuclear transfer (SCNT) using fetal fibroblasts yielded two live monkeys
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  Epigenetic modulators promoted development and pregnancy rate of SCNT embryos
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  SCNT using adult cumulus cells yielded live births of monkeys that were short-lived
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  Genetic analysis confirmed the clonal origin of the SCNT monkey offspring

Summary

Generation of genetically uniform non-human primates may help to establish animal models for primate biology and biomedical research. In this study, we have successfully cloned cynomolgus monkeys (Macaca fascicularis) by somatic cell nuclear transfer (SCNT). We found that injection of H3K9me3 demethylase Kdm4d mRNA and treatment with histone deacetylase inhibitor trichostatin A at one-cell stage following SCNT greatly improved blastocyst development and pregnancy rate of transplanted SCNT embryos in surrogate monkeys. For SCNT using fetal monkey fibroblasts, 6 pregnancies were confirmed in 21 surrogates and yielded 2 healthy babies. For SCNT using adult monkey cumulus cells, 22 pregnancies were confirmed in 42 surrogates and yielded 2 babies that were short-lived. In both cases, genetic analyses confirmed that the nuclear DNA and mitochondria DNA of the monkey offspring originated from the nucleus donor cell and the oocyte donor monkey, respectively. Thus, cloning macaque monkeys by SCNT is feasible using fetal fibroblasts.

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