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quarta-feira, julho 18, 2018

Correlated random walks of human embryonic stem cells in vitro

L E Wadkin1, S Orozco-Fuentes1, I Neganova2, G Swan1, A Laude3, M Lako2, A Shukurov1 and N G Parker1

Published 12 June 2018 • © 2018 IOP Publishing Ltd
Physical Biology, Volume 15, Number 5

Author e-mails
nick.parker@ncl.ac.uk

Author affiliations
1 School of Mathematics, Statistics and Physics, Newcastle University, Newcastle upon Tyne, United Kingdom

2 Institute of Genetic Medicine, Newcastle University, Newcastle upon Tyne, United Kingdom

3 Bio-Imaging Unit, Medical School, Newcastle University, Newcastle upon Tyne, United Kingdom

ORCID iDs

Dates
Received 2 February 2018 Accepted 25 April 2018 Published 12 June 2018




Abstract

We perform a detailed analysis of the migratory motion of human embryonic stem cells in two-dimensions, both when isolated and in close proximity to another cell, recorded with time-lapse microscopic imaging. We show that isolated cells tend to perform an unusual locally anisotropic walk, moving backwards and forwards along a preferred local direction correlated over a timescale of around 50 min and aligned with the axis of the cell elongation. Increasing elongation of the cell shape is associated with increased instantaneous migration speed. We also show that two cells in close proximity tend to move in the same direction, with the average separation of m or less and the correlation length of around 25 μm, a typical cell diameter. These results can be used as a basis for the mathematical modelling of the formation of clonal hESC colonies.

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