Uma visão CRISPR de desenvolvimento

terça-feira, fevereiro 02, 2016

A CRISPR view of development

Melissa M. Harrison 1, Brian V. Jenkins 2, Kate M. O’Connor-Giles 3,4 and Jill Wildonger 2

- Author Affiliations

1Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53706, USA;

2Biochemistry Department, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA;

3Laboratory of Genetics,

4Laboratory of Cell and Molecular Biology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA

Corresponding author:


The CRISPR (clustered regularly interspaced short palindromic repeat)–Cas9 (CRISPR-associated nuclease 9) system is poised to transform developmental biology by providing a simple, efficient method to precisely manipulate the genome of virtually any developing organism. This RNA-guided nuclease (RGN)-based approach already has been effectively used to induce targeted mutations in multiple genes simultaneously, create conditional alleles, and generate endogenously tagged proteins. Illustrating the adaptability of RGNs, the genomes of >20 different plant and animal species as well as multiple cell lines and primary cells have been successfully modified. Here we review the current and potential uses of RGNs to investigate genome function during development.


CRISPR Cas9 development genome editing genome engineering RNA-guided nuclease


Article is online at 

Freely available online through the Genes & Development Open Access option.

© 2014 Harrison et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at

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