Molecular Systems Biology 7 Article number: 550
Published online: 22 November 2011
Citation: Molecular Systems Biology 7:550
The quantitative proteomes of human-induced pluripotent stem cells and embryonic stem cells
Javier Munoz1,2,a, Teck Y Low1,2,a, Yee J Kok3,a, Angela Chin3, Christian K Frese1,2, Vanessa Ding3, Andre Choo3 & Albert J R Heck1,2
Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands
Netherlands Proteomics Center, Utrecht, The Netherlands
Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Stem Cell Group, Singapore
Correspondence to: Andre Choo3 Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Stem Cell Group, 20 Biopolis Way, Centros #06-01, Singapore 138668, Singapore. Tel.: +65 6478 8888; Fax: +65 6478 9561; Email: email@example.com
Correspondence to: Albert J R Heck1,2 Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, Utrecht 3584, The Netherlands. Tel.: +31 30 253 6797; Fax: +31 30 251 8219; Email: firstname.lastname@example.org
Received 19 July 2011; Accepted 4 October 2011; Published online 22 November 2011
aThese authors contributed equally to this workTopof page
An in-depth proteomic comparison of human-induced pluripotent stem cells, and their parent fibroblast cells, with embryonic stem cells shows that the reprogramming process comprehensively remodels protein expression levels, creating cells that closely resemble natural stem cells.
We present here a large proteomic characterization of human embryonic stem cells, human-induced pluripotent stem cells and their parental fibroblasts cell lines.
Overall, 97.8% of the 2683 quantified proteins in four experiments showed no significant differences in abundance between hESC and hiPSC highlighting the high similarity of these pluripotent cell lines.
In total, 58 proteins were found significantly differentially expressed between hiPSCs and hESCs. The observed low overlap of these proteins with previous transcriptomic studies suggests that those differences do no reflect a recurrent molecular signature.
Keywords: human embryonic stem cells; human-induced pluripotent stem cells; proteomics; quantitation