Aspectos estruturais do término de tradução no ribossomo

segunda-feira, julho 18, 2011

Structural aspects of translation termination on the ribosome

Andrei A. Korostelev1

Author Affiliations

RNA Therapeutics Institute and Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA


Translation of genetic information encoded in messenger RNAs into polypeptide sequences is carried out by ribosomes in all organisms. When a full protein is synthesized, a stop codon positioned in the ribosomal A site signals termination of translation and protein release. Translation termination depends on class I release factors. Recently, atomic-resolution crystal structures were determined for bacterial 70S ribosome termination complexes bound with release factors RF1 or RF2. In combination with recent biochemical studies, the structures resolve long-standing questions about translation termination. They bring insights into the mechanisms of recognition of all three stop codons, peptidyl-tRNA hydrolysis, and coordination of stop-codon recognition with peptidyl-tRNA hydrolysis. In this review, the structural aspects of these mechanisms are discussed.

Keywords 70S ribosome, RF1, RF2, crystal structures, release factors


1 Corresponding author.

Article published online ahead of print. Article and publication date are at
Copyright © 2011 RNA Society

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