doi:10.1038/nchembio.241
Post-reductionist protein science, or putting Humpty Dumpty back together again
Lila M Gierasch 1 & Anne Gershenson 2
Lila M. Gierasch is in the Department of Biochemistry and Molecular Biology and the Department of Chemistry, University of Massachusetts, Amherst, Amherst, Massachusetts, USA, and
Anne Gershenson is in the Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Amherst, Massachusetts, USA.
Correspondence to: Lila M Gierasch1 e-mail: gierasch@biochem.umass.edu
Correspondence to: Anne Gershenson 2 e-mail: gershenson@biochem.umass.edu
http://www.cartoonstock.com/
Abstract
In their native environments, proteins perform their biological roles in highly concentrated viscous solutions and in complex networks with numerous partners. Yet for many years, the normal practice has been to purify a protein of interest in order to characterize its structural and functional properties. In this Commentary, we discuss how protein scientists are now tackling the theoretical and methodological challenges of studying proteins in their physiological context.
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