Impacto da vizinhança genômica na evolução do transcriptoma humano-chimpanzé

quinta-feira, fevereiro 26, 2009

The impact of genomic neighborhood on the evolution of human and chimpanzee transcriptome

1.Subhajyoti De,

2. Sarah A Teichmann and

3. M. Madan Babu1


Divergence of gene expression can result in phenotypic variation, which contributes to the evolution of new species. Although the influence of trans- andcis-regulatory mutations is well known, the genome-wide impact of changes in genomic neighborhood of genes on expression divergence between species remains largely unexplored. Here, we compare the neighborhood of orthologous genes (within a window of 2Mb) in human and chimpanzee with the expression levels of their transcripts from several equivalent tissues and demonstrate that genes with altered neighborhood are more likely to undergo expression divergence than genes with conserved neighborhood. We observe the same trend when expression divergence data was analyzed from six different brain parts that are equivalent between human and chimpanzee. Additionally, we find an enrichment for genes with altered neighbourhood to be expressed in a tissue-specific manner in the human brain. These results suggest that expression divergence induced by this mechanism could have contributed to the phenotypic differences between humans and chimpanzee. We propose that, in addition to other molecular mechanisms, change in genomic neighborhood is an important factor that drives transcriptome evolution.


Received September 4, 2008.
Accepted January 14, 2009.

This manuscript is Open Access. [PDF gratuito aqui]

Copyright © 2009, Cold Spring Harbor Laboratory Press